Nanobody R&D Technology Acceleration Service Platform
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Nanobody R&D Technology Acceleration Service Platform
Yaohaibio is highly experienced in nanobody R&D and production. Relying on the strong technical platform, advanced production equipments, and pioneering technology in the nanobody full ecological expression system, Yaohaibio provides one-stop nanobody R&D and production services including strain construction, multifunctional nanobody expression and purification, and large-scale production with high efficiency and flexibility.
  • Full Ecological Recombinant Expression System

    Full Ecological Recombinant Expression System

    E.coli prokaryotic expression system, yeast eukaryotic expression system (Pichia pastoris), mammalian cell expression system

  • Diversified Nanobody Types

    Diversified Nanobody Types

    Single domain nanobody, bivalent nanobody, and multivalent nanobody

  • Mature and complete purification platform

    Mature and complete purification platform

    Complete purification platform, combined purification method, efficient and flexible

  • μg to kg

    μg to kg

    Flexible production scale

Yaohaibio has established a full ecological nanobody expression system involving E.coli prokaryotic expression system, yeast expression system (Pichia pastoris), and mammalian cell expression system. We are proficient in using various host bacterias to provide customized expression systems in high quality.

Abundant development experience in over 20 species

Flexible choice of E. coli hosts

Effective choice of expression carriers

Ample development experience in over 10 species

Flexible choice of Pichia pastoris hosts

PAOX1 methanol-induced Expression System

PGAP constitutive expression system

Affluent development experience in over 5 species of nanobody

Transient expression nanobody

Stable expression nanobody

  • E.coli prokaryotic expression system
    The E.coli expression system is the earliest developed, most widely used, and most affordable classic expression system among gene expression techniques. E.coli is characterized by clear genetic background, fast cell proliferation, high expression, good stability, and strong anti-pollution ability.
    Nanobody can be encoded by a single gene and expressed in large amounts in prokaryotic microorganisms such as E.coli by genetic engineering due to its small molecular weight and simple structure.
    Yaohaibio uses E.coli to express nanobodies in the cytoplasm or through the signaling peptide periplasmic space of over 20 types of nanobodies.
  • Yeast expression system (Pichia pastoris)
    The yeast expression system is widely used in industrial production. In addition to the clear and safe genetic background, yeast expression system harbors the advantages of both prokaryotic and eukaryotic expression systems: fast proliferation, high-density fermentation in economic media, supporting complex post-translation modification, and strong secretion expression ability, to effectively reduce downstream purification costs. Nanobody can be encoded by a single gene and expressed in large amounts in microorganisms such as yeast through genetic engineering due to its small molecular weight and simple structure.
    Yaohaibio provides high-expressed strain nanobody selection and fermentation process development services. With high-densitied fermentation, over 10 different types of nanobodies are expressed by Pichia pastoris PAOX1 induction or PGAP, and the expression amount can be up to 10 g/L or more.
  • Mammalian cell expression system
    Mammalian cell expression systems can guide the correct folding of proteins and provide various post-translation processing such as complex N-type glycosylation and accurate O-type glycosylation. Therefore, expression products are closest to natural higher biological protein molecules in terms of molecular structure, physio-chemical properties, and biological functions.
    Yaohaibio uses mammalian CHO series cells to express various nanobodies through transient transfection or the construction of stable transformants. The expression amount of nanobodies by CHO series cells can reach up to 20mg/L transient transfection and 15mg/L in stable transfection.
Nanobody expression service
Yaohaibio provides one-stop nanobody services from gene synthesis to nanobody expression, purification and production accoding to specified gene/amino acid sequences and selected host cells.
Steps Services Cycles Delivery
Construction of nanobody expression engineering strains (Pichia yeast, E.coli or mammalian cells can be selected)


1. E.coli expression system (cytoplasmic or periplasmic spatial expression)


2. Pichia yeast expression system (the methanol-induced expression system PAOX1 or the component expression system PGAP can be selected)


3. Mammalian expression system (expression of nanobody through transient or stable transfection)


E.coli: 1-2 weeks (excluding gene synthesis cycles)
Pichia pastoris: 2-3 weeks
Mammalian cell transient transfection: 1-2 weeks, stable transfection: 2 months
Test report for purified nanobodies: SDS-PAGE, SEC-HPLC (optional), RP-HPLC (optional) and CE-SDS (optional) purity analysis method
Small amount purification expression (label) Nanobody expression of the constructed engineering strain, and purification of protein expressed in 1L (labeling recommended). 2-3 weeks
Large-scale purification expression Large-scale nanobody fermentation, expression and purification 5-8 weeks
Advantages
Serving Cases

Production of different nanobodies using microorganisms

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Service case
Using microorganisms to produce different nanobodies
Using Pichia pastoris to express monovalent nanobodies
Using Pichia pastoris to express bivalent nanobodies
Using Pichia pastoris to express trivalent nanobodies

Nanobody purification system

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Service case
Nanobody purification system
Purify nanobody through affinity chromatography of Protein A.
The purity obtained by calculation is over 90% verified by PAGE.
Purify nanobody through ion chromatography.
The purity obtained by calculation is over 95% verified by PAGE.

Nanobody Quality Evaluation System

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Service case
Nanobody Quality Evaluation System
The purity of the purified nanobody can be over 95% verified by SDS-PAGE and SEC-HPLC.
The purity of the purified nanobody can be over 99% verified by SEC-HPLC.
The purity of the purified nanobody can be over 98% verified by CE-SDS.

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